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To save the economic cost and improve the performance of enterprises, this study aims to synthesize high performance immobilized penicillin G acylase (PGA) carriers with fast reaction speed, high recovery rate of enzyme activity and good reusability through corresponding theoretical guidance and experimental exploration.

A diblock resin was synthesized by reversible addition-fragmentation chain transfer polymerization method using N, N-diethylacrylamide (DEA) and β-hydroxyethyl methacrylate (HEMA) as functional monomers poly(N, N-diethylacrylamide)-b-poly(β-hydroxyethyl methacrylate) (PDEA-b-PHEMA) was obtained, and the effect of the ratio of DEA and HEMA on the activity of PGA was investigated, and the appropriate block ratio of DEA and HEMA was obtained. After that, the competitive rate of HEMA and glycidyl methacrylate (GMA) under the carrier preparation conditions was investigated. Based on the above work, a thermosensitive resin carrier PDEA-b-PHEMA-b-P(HEMA-co-GMA) with different target distances was synthesized, and the chemical structures and molecular weight of copolymers were investigated by hydrogen NMR (¹H NMR).

The lower critical solution temperature of the resin support decreases with the increase of the monomer HEMA in the random copolymerization; the catalytic performance study indicated that the response rate of the immobilized PGA is fast, and the recovery rate of the enzyme activity of the immobilized PGA varies with the distance between the targets. When the molar ratio of HEMA to GMA in the resin block is 8.15:1 [i.e. resin PDEA100-b-PHEMA10-b-P(HEMA65-co-GMA8)], the activity recovery rate of immobilized PGA can reach 50.51%, which was 15.49% higher than that of pure GMA immobilized PGA.

This contribution provides a novel carrier for immobilizing PGA. Under the optimal molar ratio, the enzyme activity recovery could be up to 50.51%, which was 15.49% higher than that of PGA immobilized on the carrier with nonregulated distance between two immobilization sites.

In today's complex and rapidly changing business environment, cross-boundary growth is increasingly critical to the survival or even success of organizations. The purpose of this study is to examine the forming mechanism of firm’s cross-boundary growth by integrating the two important antecedent factors of performance pressure and managerial discretion into a united framework and theoretically analyze the direct role of performance pressure on firm’s cross-boundary growth as well as reveal the moderating role of managerial discretion. Also, the authors select listed manufacturing companies in China as samples to empirically test the research hypotheses.

The authors design a multiple regression model to perform empirical analysis by using a panel of 4,002 year-observations in 1,334 listed manufacturing companies between 2013 and 2016. The sample data sources mainly come from the Wind Database, which is mainland China's leading financial database and software services provider. The hypotheses proposed are tested by adopting a panel data set of the listed manufacturing companies of China.

Empirical results show that performance pressure has a positive effect on the cross-industry growth and cross-domestic regional growth but a negative effect on the cross-international regional growth, and managerial discretion has a different moderating effect. Specifically, capital intensity strengthens the positive effect of performance pressure on cross-industry growth but weakens the negative effect of performance pressure on cross-international regional growth. State ownership enhances the positive effect of performance pressure on cross-domestic regional growth but decreases the negative effect of performance pressure on cross-international regional growth. CEO duality increases the negative impact of performance pressure on cross-international regional growth.

This study provides several implications for top executives, including how to dialectically consider the double-edged effect of performance pressure on cross-boundary growth of firms, create an appropriate environments of managerial discretion and design the types of cross-boundary growth strategies that top executives can follow in the volatility, uncertainty, complexity and ambiguity era.

Although the relevant literature highlights the importance of performance pressure, it has not been related to the cross-boundary growth of firms. This paper makes an incremental contribution to the literature on the forming mechanisms of firm’s cross-boundary growth by providing an important perspective of performance pressure to firm growth determinants and taking into account the moderating role of managerial discretion.

As the excessive lifting force can lead to catwalk rollover and well site accidents, the lifting process boundary conditions and structural parameters have a significant effect lifting force, it is important to analysis the structural parameters on the maximum lifting force in the lifting process of power catwalk.

A new model is proposed to analyze the influence of structure parameters on its lifting force for lifting power catwalk in this paper, and the geometric and dynamic equations are established according to the different boundary conditions in different stages. In addition, the establishment of dynamics equations is based on D'Alembert's principle. To solve the model, dynamic analysis software is developed, which uses c # call MATLAB to solve the geometric and dynamic equations. The maximum lifting force is analyzed and optimized according to the software, the influence of structural parameters on the maximum lifting force is obtained and the correctness of the optimization is proved by experiments.

The best value of offset e is 0. The length of L22 should as small as possible while the installation size of the end of the conveying arm are guaranteed. The length of L1 should as small as possible while ensuring the not exceed the maximum value. The maximum lifting force remain the same in the second phase, the maximum lifting force decreases with the increase of Lcp, Lcpshould as small as possible. The maximum pressure of the hydraulic oil dropped by an average of 13.62% under optimized parameters.

This paper provides a theoretical basis for the selection of hydraulic winch, which also provides the theoretical basis and data support for the design and optimization of the structural parameters of the power catwalk.

This research has industrial applications in SJ Petroleum Machinery CO.LTD, SINOPEC (China) .CANRIG, North Rig, TESCO, Sichuan HONGHUA petroleum equipment CO.LTD of CNPC., Baoji Oil field Machinery CO.LTD, SJ Petroleum Machinery Co. LTD of SINOPEC, Yantai Jereh Oilfield Services Group CO.LTD, Nanyang clips oil equipment (group) CO. LTD, etc are the likely users.

A new model is proposed to analyze the lifting force of lifting power catwalk. The model takes into account the inertia force of the structure, development of dynamics software and analysis and optimization of structural parameters. The maximum lifting force is analyzed and optimized according to the software, the influence of structural parameters on the maximum lifting force is obtained and the correctness of the optimization is proved by experiments.

This paper aims to explore the relationship between ethical self-fashioning and citizenship practices in the ongoing revival of “Chinese Traditional Culture” pursued in tandem by the party-state and by private actors in present-day China.

Adopting an anthropological approach, the author draws from three sets of resources: (1) research literature on China’s political history and key texts of early Chinese thought, (2) contemporary state discourses on citizen formation, and (3) participant observation notes and interviews with organizers and followers of the Wu-Wei School (a pseudonym). The author conducts a textual analysis of primary and secondary literature and a critical discourse analysis of the ethnographic data and examines emerging themes.

Firstly, the author identifies a crucial dimension in the historical and cultural roots of Chinese citizenship practices: an enduring conception that binds individual ethical self-improvement with socio-political flourishing. Secondly, examining contemporary state discourses on “citizen quality” and “reviving China’s outstanding traditional culture”, the author showcases how party-state authorities call on individuals to self-reform for national rejuvenation. Thirdly, the author investigates how members of the Wu-Wei School construe their individual pursuits of ethical self-improvement as significant for societal progress.

Based on these findings, the author demonstrates the ways in which autochthonous conceptions of Chinese citizenship give a central place to private acts of self-fashioning. The author argues that the entanglement between individual ethics and citizenship practices constitutes a crucial but largely understudied dimension of Chinese citizenship.

Under the repeated action of the construction load, opening deformation and disturbed deformation occurred at the precast box culvert joints of the shield tunnel. The objective of this paper is to investigate the effect of construction vehicle loading on the mechanical deformation characteristics of the internal structure of a large-diameter shield tunnel during the entire construction period.

The structural response of the prefabricated internal structure under heavy construction vehicle loads at four different construction stages (prefabricated box culvert installation, curved lining cast-in-place, lane slab installation and pavement structure casting) was analyzed through field tests and ABAQUS (finite element analysis software) numerical simulation.

Heavy construction vehicles can cause significant mechanical impacts on the internal structure, as the construction phase progresses, the integrity of the internal structure with the tunnel section increases. The vertical and horizontal deformation of the internal structure is significantly reduced, and the overall stress level of the internal structure is reduced. The bolts connecting the precast box culvert have the maximum stress at the initial stage of construction, as the construction proceeds the stress distribution among the bolts gradually becomes uniform.

This study can provide a reference for the design model, theoretical analysis and construction technology of the internal structure during the construction of large-diameter tunnel projects.

In order to accomplish real‐time alignment of Shipborne strapdown inertial navigation system (SINS) on moving bases, a novel solution method of utilizing neural networks for rapid transfer alignment of Shipborne SINS was investigated.

The system error state equations and measurement equations of the Shipborne transfer alignment were established. Based on the nonlinear and time‐variant SINS model on moving bases, a neural network learning algorithm based on Kalman filtering was presented, and the methods of constructing and training of neural networks input‐output sample pairs suitable for Shipborne SINS were proposed.

Velocity and attitude errors between the master and slave inertial navigation system (INS) are chosen as network's inputs, and the information of sample pairs is affluent, which can advance the stability and generalization of the neural networks. The neural networks algorithms based on Kalman filtering not only have the self‐learning ability, but also remain recursive optimal estimation capability of Kalman filtering. Through the introducing of the local level trajectory frame, the trained neural networks can be independent on a ship heading, and only dependent on the relative position errors between master with slave INS and the inertial sensor errors.

This article presents an innovative solution method of utilizing neural networks for rapid transfer alignment of Shipborne SINS.

Smart contracts are written in high-level programming languages, compiled into Ethereum Virtual Machine (EVM) bytecode, deployed onto blockchain systems and called with the corresponding address by transactions. The deployed smart contracts are immutable, even if there are bugs or vulnerabilities. Therefore, it is critical to verify smart contracts before deployment. This paper aims to help developers effectively and efficiently locate potential defects in smart contracts.

GethReplayer, a smart contract testing method based on transaction replay, is proposed. It constructs a parallel transaction execution environment with two virtual machines to compare the execution results. It uses the real existing transaction data on Ethereum and the source code of the tested smart contacts as inputs, conditionally substitutes the bytecode of the tested smart contract input into the testing EVM, and then monitors the environmental information to check the correctness of the contract.

Experiments verified that the proposed method is effective in smart contract testing. Virtual environmental information has a significant effect on the success of transaction replay, which is the basis for the performance of the method. The efficiency of error locating was approximately 14 times faster with the proposed method than without. In addition, the proposed method supports gas consumption analysis.

This paper addresses the difficulty that developers encounter in testing smart contracts before deployment and focuses on helping develop smart contracts with as few defects as possible. GethReplayer is expected to be an alternative solution for smart contract testing and provide inspiration for further research.

For coastal bridges, the ability to recover traffic functions after the earthquake has crucial implications for post-disaster reconstruction, which makes resilience become a significant index to evaluate the seismic behavior. However, the deterioration of the material is particularly prominent in coastal bridge, which causes the degradation of the seismic behavior. As far, the research studies on resilience of coastal bridges considering multiple degradation factors and different disaster prevention capability are scarce. For further evaluating the seismic behavior of coastal bridge in the long-term context, the seismic resilience is conducted in this paper with considering multiple durability damage.

The fuzzy theory and time-varying fragility analysis are combined in this paper to obtain the life-cycle resilience of coastal bridges.

The results show that durability damage has a remarkable impact on the resilience. After 100 years of service, the seismic resilience of bridge with poor disaster-prevention capability has greatest reduction, about 18%. In addition, the improvement of the disaster prevention capability can stabilize the resilience of the bridge at a higher level.

In this paper, the time-varying fragility analysis of case bridge are evaluated with considering chloride ion erosion and concrete carbonization, firstly. Then, combining fuzzy theory and fragility analysis, the triangular fuzzy values of resilience parameters under different service period are obtained. Finally, the life-cycle resilience of bridge in different disaster prevention capability is analyzed.

This paper aims to investigate the distributed coordinated fuzzy tracking problems for multiple mechanical systems with nonlinear model uncertainties under a directed communication topology.

The dynamic leader case is considered while only a subset of the follower mechanical systems can obtain the leader information. First, this paper approximates the system uncertainties with finite fuzzy rules and proposes a distributed adaptive tracking control scheme. Then, this paper makes a detailed classification of the system uncertainties and uses different fuzzy systems to approximate different kinds of uncertainties. Further, an improved distributed tracking strategy is proposed. Closed-loop systems are investigated using graph theory and Lyapunov theory. Numerical simulations are performed to verify the effectiveness of the proposed methods.

Based on fuzzy control and adaptive control theories, the desired distributed coordinated tracking control strategies for multiple uncertain mechanical systems are developed.

Compared with most existing literature, the proposed distributed tracking algorithms use fuzzy control and adaptive control techniques to cope with system nonlinear uncertainties of multiple mechanical systems. Moreover, the improved control strategy not only reduces fuzzy rules but also has higher control accuracy.

The main purpose of this paper is to enhance the control performance of the robotic arm by the controller of fuzzy neural network (FNN).

The robot system has characters of high order, time delay, time variation and serious nonlinearity. The classical PID controller cannot achieve satisfactory performance in control of such a complex system. This paper combined the fuzzy control with neural networks and established the FNN controller and applied it in control of the robot.

The experimental results showed that the FNN controller had excellent performances in position control of the rehabilitation robotic arm such as fast response, small overshoot and small vibration.

This work is focused on the static FNN algorithm by updating the second and fifth layers of the membership functions. The performance can be improved further if the third layer (reasoning layer) can be updated online.

Based on a hierarchical structure of the FNN controller, this paper designed the FNN controller and applied it in control of the rehabilitation robot driven by pneumatic muscles.