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The assessment of ecosystem services (ES) in urban areas can be an important policy instrument for both strategic directives at national and regional levels and concrete actions at municipality level. A unifying underpinning framework that combines different administrative levels through a common, consistent basis would greatly facilitate the mainstreaming of such an instrument. Three key concepts underpin this framework: urban ecosystems, nature-based solutions (NBS), and ES. First, NBS are acknowledged as the ecological assets that in the urban context can provide ES, meant as complex processes made possible through the existence of natural and seminatural systems at various scales. Second, the classification of urban ecosystems needs to be consistent with the treatment of urban areas in ecosystem accounting and with NBS levels of intervention and primary objectives. Third, by serving societal challenges, it is important to clearly frame the strategic drivers of change: whether it concerns management practices of current land use or radical conversions in land use. Finally, once such a framework will be in place, it can facilitate sustainability assessment in urban ecosystems by quantifying the presence of NBS, whose creation/maintenance assure the delivery of ES. The purpose of this chapter is to theoretically frame an ES-based approach, but this is only a first step. Many more steps are required on to concretely put this framework in practice at different administrative levels, such as strategic planning and policy setting (at national level), and urban, peri-urban, and coastal development (at municipal level).