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Bacterial exopolysaccharides (eps) have fascinating chemical compositions, properties and structures which could be used in the modification of natural fibres. Bacterial eps have therefore been used to modify plant cellulose fibre surface and impart desired properties. The purpose of this paper is therefore to investigate the influence of gin trash cultured bacteria eps on the physical and structural properties of cotton fibres.

Gin trash soil sample was collected from a ginnery in Kenya, and physiochemical and microbial characterization was done. The soil sample was then fermented for 24 h before being used to treat raw cotton fibres at varied conditions of temperature, pH and treatment time periods. Physical and structural properties of the treated fibres were then determined using USTER HVI-1000 M700, Fourier transform infrared, scanning electron microscope (SEM) and X-ray diffraction (XRD) and compared with those of the raw fibres.

The bacteria broth treated fibres were found to have increased in strength, spinning consistency index, elongation and fineness by 25.44, 24.30, 11.70 and 3.60%, respectively. The variations were attributed to interactions of bacterial eps with cotton cellulose through hydrogen bonding. SEM and XRD analysis revealed an increase in fibre surface roughness and crystallinity, respectively.

Bacterial eps have been used to modify plant cellulose fibre surface and impart desired properties. Eps producing bacteria have been isolated from different habitats such as saline water, soil samples, food wastes and petroleum-contaminated soil. To the best of the authors’ knowledge, bacterial eps cultured from gin trash soil sample for modification of cotton fibres have however not been previously done, hence the originality of the current study.

The purpose of this paper is to investigate the efficacy of bacterial exopolysaccharides (Eps) in reactive black 5 (RB5) textile dye wastewater bioremediation.

The Eps were produced by bacteria isolated from cotton gin trash soils collected from different cotton-growing regions in Kenya for comparison purposes. A broth medium reconstituted using molasses was assessed for its capacity to produce the Eps. RB5 textile dye wastewater was optimized for dye removal under different temperatures, times and molasses concentrations. Dye removal was studied by Lovibond-Day Light Comparator, UV–Vis spectrophotometer and FTIR.

It was found that cotton gin trash soils contained Eps-producing bacteria. Three of the Eps studied were found to have the capacity to remove at least 80% of the dye from the wastewater.

This research did not assess the efficacy of the RB5 dye removal from the wastewater by mixtures of the Eps.

Bioremediation of textile dye wastewater with Eps produced by bacteria cultured from cotton gin trash soil is significant because it will offer an effective and cleaner alternative to the chemical coagulants.

Alternative treatment of textile wastewater with the Eps would result in safer water being released into the water bodies as opposed to the chemically treated wastewater that contains remnant chemicals.

Research on the use of Eps produced by bacteria isolated from cotton gin trash soils for removal of RB5 dye from textile wastewater has not been done before.